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Animal Hospital

Second Floor

Second Floor event for Animal Hospital Roblox, ran 2026-09-05โ€“2026-09-07. The event window has closed but the new floor, SEWERS area, Laboratory and DNA Sampler remain in the game.

By Animal Hospital Guide ยท Site Maintainer: CT

Last updated:

Ended๐Ÿ“… 2026-09-05 โ€“ 2026-09-07
Official Event Page โ†—

๐ŸŽฌ Official Gameplay & Video Walkthrough

FULL GUIDE To The SECOND FLOOR UPDATE In Animal Hospital! (New Lore, Secret Areas, Lab) Roblox

๐ŸŽ What it added

Confirmed Highlights
Second floor: a brand-new floor of the hospital with SEWERS and unexplored rooms
Laboratory: brew potion vials to use on patients or yourself
DNA Sampler tool: collect DNA samples from patients in beds
Board displaying all stackable effects
New hidden location and new lore elements
New achievements and four new skins
Bug fixes and a leaderboard reset
Second Floor artwork

๐Ÿ“– What this update delivered

The Second Floor event for Animal Hospital Roblox ran 2026-09-05โ€“2026-09-07. The event window itself has closed, but everything it introduced is permanent content, so this is not a recap of a limited-time mode โ€” it is how you access the second floor, the SEWERS area and the Laboratory today.

The headline addition is the Laboratory: use the DNA Sampler on a patient to collect a sample, sequence it, then combine three DNA ingredients to brew a vial. Pink serums are thrown at patients and NPCs, while green serums are drunk by you.

The update also added a board that lists every stackable effect, a new hidden location with new lore elements, new achievements and four new skins, plus bug fixes and a leaderboard reset.

FAQ

Is the Second Floor update live right now?

The event window ran from 2026-09-05 through 2026-09-07 and has now closed. The content it introduced โ€” the second floor, the SEWERS area, the Laboratory and the DNA Sampler โ€” is still playable in Animal Hospital.

How do I reach the second floor?

The second floor unlocks after you complete your first ambulance visit. If you are playing in skip-tutorial mode, it is available from the start of the run.

What is the Laboratory for?

The Laboratory is the headline feature of the update. Use the DNA Sampler on a patient to collect a sample, sequence it, then mix three DNA ingredients to brew a vial. Pink serums are thrown at patients and NPCs; green serums are drunk by you. See the Laboratory & DNA Guide for the full recipe list.

Do I need to have played during the event window to get the second floor?

No. The event dates only marked when the content was released. The second floor, SEWERS area, Laboratory and DNA Sampler are all still in the game, so a player starting today has exactly the same access as someone who logged in during September 5โ€“7.

What is the DNA Sampler used for?

The DNA Sampler is the tool that feeds the Laboratory. Use it on a patient in a bed to collect a DNA sample, then sequence that sample and combine three DNA ingredients into a brewable vial. Without the sampler you cannot produce any serum.

What do the pink and green serums do differently?

The delivery method, not just the effect, distinguishes them. Pink serums are thrown at patients and NPCs, making them the tool for influencing someone else. Green serums are drunk by you, so they are self-applied. Some serums are only worth brewing when you consider which of the two you actually need.

Every Official Animal Hospital Event

All dated events Roblox has listed for this game, newest first โ€” the closed windows included, because the content each one shipped is still playable in the game today.

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